Application of T-SPOT .TB and TB-PCR detection in extra pulmonary tuberculosis
10.3969/j.issn.1673-4130.2015.14.019
- VernacularTitle:结核感染 T 细胞斑点试验与荧光定量 PCR在肺外结核诊断中的应用
- Author:
Chengyong WANG
;
Shihu PAN
;
Min LIU
;
Jie LIU
;
He WU
- Publication Type:Journal Article
- Keywords:
T-SPOT .TB;
polymerase chain reaction;
tuber culosis
- From:
International Journal of Laboratory Medicine
2015;(14):1998-1999
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the significance of T‐SPOT .TB and real‐time quantitative Polymerase Chain Reaction (PCR) in the diagnosis of extrapulmonary tuberculosis .Methods Among the 125 cases of extrapulmonary tuberculosis group ,all the 125 cases were performed in accordance with the acid fast bacilli smear ,TB‐PCR and T‐SPOT .TB detection .87 cases of Non‐tuberculosis disease group were detected .Results Among 125 cases of extrapulmonary tuberculosis ,4 cases were positive for acid fast bacilli smear and the positive rate was 3 .2% ;57 cases were positive byTB‐PCR and the positive rate was 45 .6% ;96 cases were positive by T‐SPOT .TB and the positive rate was 76 .8% .wo methods were detected in104 cases of positive patients ,the positive rate was 83 .2% .3 cases were positive by TB‐PCR and the positive rate was 3 .4% in 87 cases with non tuberculosis disease group . 4 cases were positive by T‐SPOT .TB and the positive rate was 4 .6% .Conclusion Here is an important practical value in the diag‐nosis of T‐SPOT .TB detection technology in extra pulmonary tuberculosis ,and TB‐PCR joint detection could further improve the detection rate of pulmonary tuberculosis .
