Comparison of different methods for detecting mycoplasma pneumoniae
10.3969/j.issn.1673-4130.2015.14.048
- VernacularTitle:不同方法检测肺炎支原体的比较
- Author:
Haiping LI
;
Kun DU
- Publication Type:Journal Article
- Keywords:
mycoplasma pneumoniae;
fluorescence quantitative polymerase chain reaction;
enzyme-linked immunosorbent assay
- From:
International Journal of Laboratory Medicine
2015;(14):2061-2062
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the sensitivity and specificity of different methods for the detection of mycoplasma pneu‐moniae .Methods The serum and throat swab specimens were collected from 199 pneumonia patients .ELISA and real‐time fluores‐cence quantitative polymerase chain reaction (FQ‐PCR) assays were used to detect the serum mycoplasma pneumoniae IgM antibod‐y and DNA .Results Among 199 pneumonia patients ,61 cases were diagnosed as mycoplasma pneumoniae pneumonia according to the diagnosis standard .The sensitivity and specificity of ELISA and FQ‐PCR for detecting mycoplasma pneumoniae were 86 .9%and 96 .7% ,and 78 .3% and 97 .1% ,respectively .Conclusion The sensitivity and specificity of FQ‐PCR for detecting mycoplasma pneumoniae are higher than those of ELISA .
