Cytogenetic abnormalities detected by fluorescence in situ hybridization panel in chronic lymphocytic leukemia

VernacularTitle:成套荧光原位杂交探针在慢性淋巴细胞白血病染色体异常检测中的应用
Author: Yi YING ; Xiaoyan CHEN ; Qinghua DU ; Hanping WANG ; Jianjin XIE ; Xiaowei CHEN
Publication Type:Journal Article
Keywords: Leukemia,lymphocytic,chronic; Fluorescence in situ hybridization; Chromosome
From: Journal of Leukemia & Lymphoma 2014;23(7):416-419
CountryChina
Language:Chinese
Abstract: Objective To investigate the significance of fluorescence in situ hybridization (FISH) panel in detecting cytogenetic abnormalities in patients with chronic lymphocytic leukemia (CLL).Methods A panel of FISH probes [D13S25 (13q14.3),RB1 (13q14),ATM(1 1q22.3),CSP12(12p1 1.1-12q1 1.1) and p53 (17p13.1)] were performed in 21 cases with CLL.The cytogenetic features in correlation with clinical manifestation,other laboratory tests and prognosis were analyzed.Results Cytogenetic abnormalities were found in 13 of 21 patients with CLL (61.90 ％).The most frequent abnormality was del(13q14) (42.86 ％),followed by trisomy 12 (14.29 ％),del(11q22) (9.52 ％) and del(17p13) (9.52 ％).There was no significant relationship among cytogenetic abnormalities and sex,binet stages,expression of CD38,level of lactate dehydrogenase.Conclusion FISH with probe panel is a rapid,sensitive and accurate technique for detection of cytogenetic abnormalities in patients with CLL.