Effects of Catalpol on Apoptosis andβ-Tubulin of Rabbit Sinoatrial Node Cells in Vitro Injured by Simulated Ischemia Reperfusion
10.3969/j.issn.1005-5304.2015.08.016
- VernacularTitle:梓醇对模拟缺血再灌注损伤兔窦房结细胞凋亡及细胞骨架β-微管蛋白的影响
- Author:
Ruxiu LIU
;
Jie PENG
;
Yu LIU
;
Jinfeng LIU
;
Yanli WANG
- Publication Type:Journal Article
- Keywords:
catalpol;
atrionector cell;
ischemia reperfusion;
apoptosis;
β-tubulin;
rabbits
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;22(8):59-62
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effects of catalpol, the effective component of Rehmanniae Radix, on atrionector cell apoptosis andβ-tubulin in vitro rabbit injured by simulated ischemia reperfusion;To explore the mechanism of treating sick sinus syndrome.MethodsAtrionector cells were collected from newborn rabbits. Cells were divided into 5 groups:normal group, model group, catalpol high, medium and low dose groups. Anoxia and aglycaemia were established to simulate ischemia. Atrionector cellular damage models were established by recovering the supply of oxygen and sugar. Normal control group and model group were given the same volume of culture medium, while catalpol high, medium and low dose groups were given medicine with relevant concentrations (100, 20, 10μg/mL, respectively). ELISA, FCM, laser scanning confocal microscopy were used to observe apoptosis rate andβ-tubulin of atrionector in each group.Results Apoptosis rate of the model group was obviously higher than the normal group (P<0.01), andβ-tubulin cleavage was obvious. Apoptosis rate in catalpol high, medium and low dose groups were significantly lower than that of the model group (P<0.01);β-tubulin structure were significantly more complete compared with the model group;the fluorescence intensity was significantly higher than that of model group (P<0.01).Conclusion Catalpol can inhibit atrionector cellular apoptosis caused by simulated ischemia reperfusion. Its protective effects on atrionectorβ-tubulin may be the mechanism of the treatment of Rehmanniae Radix for sick sinus syndrome.
