Content Determination of Paeoniflorin and Icariin inQixue Shuangbu Oral Liquid by HPLC

VernacularTitle:HPLC测定气血双补口服液中芍药苷与淫羊藿苷含量
Author: Haixiang REN ; Liye QIAO ; Lu HUANG ; Jia JIA ; Yin LU ; Hua SU
Publication Type:Journal Article
Keywords: Qixue Shuangbu Oral Liquid; paeoniflorin; icariin; HPLC; content determination
From: Chinese Journal of Information on Traditional Chinese Medicine 2015;22(8):80-82
CountryChina
Language:Chinese
Abstract: Objective To improve the quality standard of Qixue Shuangbu Oral Liquid;To provide guarantee for the quality of oral liquids.Methods HPLC was used to simultaneously determine the contents of paeoniflorin and icariin in the oral liquid. The determination was performed on a Agiltnt TC-C18 (2) column (250 mm × 4.6 mm, 5μm) with the mobile phase of acetonitrile and 0.1% H3PO4 gradient mode. The flow rate was 1.0 mL/min;the column temperature was set at 25℃; the detection wavelength was 232 nm.Results The linear ranges of paeoniflorin and icariin were obtained between 0.542-8.677μg (r=0.999 9) and 0.185-2.963μg (r=0.999 9), with the average recoveries (n=6) of 99.29% (RSD=1.06%) and 98.91% (RSD=1.97%), respectively.Conclusion This method is accurate and feasible, which can be used conveniently in quality control ofQixue Shuangbu Oral Liquid.