Effect of low-expressed MK on proliferation, migration and angiogenesis of HUVECs cells in human breast cancer cell line MDA-MB-231
10.13315/j.cnki.cjcep.2015.07.003
- VernacularTitle:干扰人乳腺癌MDA-MB-231细胞 MK表达对内皮细胞增殖、迁移及脉管形成的影响
- Author:
Dongmei LIU
;
Yongping WU
;
Qingling WANG
- Publication Type:Journal Article
- Keywords:
breast neoplasm;
cancer cell;
MK;
HUVECs;
proliferation;
migration;
angiogenesis
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(7):729-733
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To observe the effect of MK on proliferation, migration and angiogenesis of human umbilical vein endothelial HU-VECs cells and to explore the role of MK in tumor angiogenesis. Methods siRNA targeting MK was used to downregulate MK expres-sion in human breast cancer cell line MDA-MB-231. Then, the experiment was divided into three groups: the untreated group, the empty vector transfection group and MK gene interference group. CCK-8 assay was used to detect the endothelial cells proliferation, tr-answell method was for detection of endothelial cell migration numbers, and matrigel in vitro small tube formation assay was used to sur-vey the state of tube formation. Expression of MK in siRNA transfection was identified by RT-PCR and Western blot. Results The MK gene interference group showed lower cell proliferation activity, less number of migration of cells and tube formation than the other two groups (P<0. 05). Conclusion Low-expressed MK in human breast cancer cell line MDA-MB-231 can inhibit proliferation, mi-gration and angiogenesis of endothelial cells, which shows that MK may play an important role in tumor angiogenesis.
