Comparison of real-time PCR method with Sanger sequencing for detection of BRAF muta-tion in papillary thyroid carcinoma

VernacularTitle:比较实时荧光 PCR 法与 Sanger 测序法检测甲状腺乳头状癌BRAF基因突变
Author: Tian QIU ; Wenting HUANG ; Lei GUO ; Haizhen LU ; Yun LING ; Ling SHAN ; Wenbin LI ; Ning Lü ; Jianming YING
Publication Type:Journal Article
Keywords: thyroid neoplasm; papillary carcinoma; BRAF gene mutation; real-time PCR; Sanger sequencing
From: Chinese Journal of Clinical and Experimental Pathology 2015;(7):756-758
CountryChina
Language:Chinese
Abstract: Purpose To investigate the positive rate and concordance rate of BRAF mutation in papillary thyroid carcinoma detected by real-time PCR method and Sanger sequencing. Methods 312 papillary thyroid carcinomas patients were enrolled in this study. Real-time PCR method and Sanger sequencing were performed to detect BRAF gene mutations. The frequency of BRAF mutation and the concordance of two methods were analyzed. Results BRAF mutation was detected in 65. 4% (204/312) and 63. 8% (199/312) of 312 papillary thyroid carcinoma samples by using real-time PCR method and Sanger sequencing, respectively. There was no significant correlation between BRAF gene mutations and patients’ gender. There was significant correlation between BRAF gene mutations and patients’ age. The overall concordance between real-time PCR method and Sanger sequencing for BRAF mutation detection was 98. 4%. Conclusion Real-time PCR method provides an effective method in BRAF gene mutation detection.