HPLC Characteristic Chromatogram and HPLC-MS/MS Analysis ofKanglao Qingfei Granules

VernacularTitle:抗痨清肺颗粒HPLC特征图谱及液质联用分析
Author: Lin TANG ; Chunqing LUO ; Xinhua XIA ; Jianguo ZENG ; Yanke LIU
Publication Type:Journal Article
Keywords: Kanglao Qingfei Granules; characteristic chromatogram; HPLC; HPLC-MS/MS
From: Chinese Journal of Information on Traditional Chinese Medicine 2015;22(8):88-91
CountryChina
Language:Chinese
Abstract: Objective To establish HPLC characteristic chromatogram ofKanglao Qingfei Granules.Methods HPLC analysis of samples was performed on Kromasil C18 column (4.6 mm × 250 mm, 5μm), with acetonitrile-1% glacial acetic acid as the mobile phase of gradient elution (0-50 min, 5%→15% acetonitrile;50-70 min, 15%→25% acetonitrile;70-80 min, 25%→40% acetonitrile;80-90 min, 40%→65% acetonitrile, 90-120 min, 65%→95% acetonitrile);the volume flow rate was 1.0 mL/min;detection wavelength was set at 290 nm;column temperature was 30℃. Chromatographic peaks were identified by HPLC-MS/MS method.Results The similarity degrees of 10 batches of samples were all greater than 0.995, and 13 chromatographic peaks were determined as common characteristic peaks, of which 10 peaks were confirmed in the source attribution and 8 peaks were identified in chemical component.Conclusion The established HPLC characteristic chromatogram can be used for the quality control ofKanglao Qingfei Granules.