Study of a multiplex PCR method for the detection of three Diarrheagenic Escherichia coli
10.3969/j.issn.1673-4130.2015.08.008
- VernacularTitle:多重 PC R快速检测3种致泻性大肠埃希菌方法的建立
- Author:
Dong YAO
;
Rusheng ZHANG
;
Xinhua OU
- Publication Type:Journal Article
- Keywords:
diarrheagenic Escherichia coli;
multiplex polymerase chain reaction;
rapid detection
- From:
International Journal of Laboratory Medicine
2015;(8):1032-1033
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a multiplex polymerase chain reaction (PCR) method for rapid detection of three kinds of di‐arrheagenic Escherichia coli(EPEC ,EIEC ,EHEC)simultaneously .Methods The eae gene of EPEC ,ipaH gene of EIEC and stx1 gene of EHEC were selected to design primers ;the reaction system and condition were adjusted to optimize the multiplex PCR sys‐tem .Results The target gene fragments were amplified correctly with these primers .The three target bacteria could be detected at the same time by multiplex PCR .Conclusion A rapid multiplex PCR system were successfully established for detection of three di‐arrheagenic Escherichia coli ,and this system could be suitable for rapid screening in food safety .
