Sequence analysis of a novel HLA-A?01 ∶130 allele and modeling of three-dimensional structure of HLA molecule
10.3969/j.issn.1000-484X.2015.04.016
- VernacularTitle:新等位基因HLA－A?01∶130的序列分析及HLA分子三维结构模拟
- Author:
Xin LI
;
Juan DING
;
Ling HOU
;
Xin WANG
;
Tingyu YAN
;
Jihong LI
;
Chunyan ZHANG
;
Suzhen ZHAO
;
Ying LIU
;
Jie LIU
- Publication Type:Journal Article
- Keywords:
Typing;
A novel allele;
Sequencing Based Typing;
Modeling of three-dimensional structure
- From:
Chinese Journal of Immunology
2015;(4):514-516
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To confirm the novel allele HLA-A*01∶130 and analyzed the nucleotide sequence of the abnormal reaction pattern.Methods: The HLA typing of sample DNA was performed by PCR-SBT.The ambiguous novel HLA allele was confirmed with single stranded SBT method,then DNA sequencing was performed to identify the difference between the novel allele and HLA-A?01:66 allele.Finally, it was modeled by Swiss-Model to three-dimensional structure of HLA Molecule.Results: The novel allele was not the same with all known HLA-A allele sequence.After analysis,there was one nucleotide differed from the A?01:66 at position 368 where A→G( codon 99 TAT→TGT) resulting in a coding change,99 Tyr was changed to Cys.The amino acid substitution at residue 99 the HLA polypeptide was located in a beta-sheet of antigenic peptide-binding region.Conclusion: The allele is a novel allele that has now been officially named as HLA-A*01∶130 by the World Health Organization( WHO) HLA Nomenclature Committee.
