Effects of different doses of puerarin on osteoblastsin vitro
10.3969/j.issn.2095-4344.2015.11.004
- VernacularTitle:不同浓度葛根素对体外培养成骨细胞的影响
- Author:
Shuzhong LIN
;
Jun LIU
- Publication Type:Journal Article
- Keywords:
Drugs,Chinese Herbal;
Osteoblasts;
Alkaline Phosphatase;
Cel Proliferation
- From:
Chinese Journal of Tissue Engineering Research
2015;(11):1658-1662
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies have reported that puerarin can reduce bone resorption and promote bone formation. OBJECTIVE: To further explore the effects of puerarin at different doses on the proliferation of osteoblasts culturedin vitro. METHODS: Osteoblasts isolated from the cranium of newborn rats were culturedin vitro, and then passage 3 cels were cultured in DMEM medium with the presence of 0, 20, 40, 80 and 100 μmol/L puerarin+10% fetal bovine serum for 48 hours. The viability of osteoblasts, alkaline phosphatase activity and mineral node formation were determined using MTT, alkaline phosphatase kit and alizarin red staining, respectively. RESULTS AND CONCLUSION:The viability of osteoblasts treated with puerarin at either 40 or 80 μmol/L was significantly higher than that of the control group. Alkaline phosphatase activity and the number of mineral modules were significantly increased in osteoblasts cultured with puerarin at 40 or 80 μmol/L when compared with the untreated cels. These findings demonstrate that puerarin is able to promote osteoblast proliferation in vitro.
