Effects ofJiegu Qili Tablet on proliferation and mineralization of MC-3T3 cells
10.3969/j.issn.2095-4344.2015.11.011
- VernacularTitle:成骨细胞增殖和钙盐沉积能力：在接骨七厘片药物溶液中的反应
- Author:
Mingzhi YAO
- Publication Type:Journal Article
- Keywords:
Osteoblasts;
Cel Proliferation;
Fracture Healing;
Drugs,Chinese Herbal
- From:
Chinese Journal of Tissue Engineering Research
2015;(11):1694-1698
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Jiegu Qili Tablet is a compound Chinese medicine which can promote fracture healing, but its mechanism is stil unclear. OBJECTIVE: To observe the effects ofJiegu Qili Tablet on the proliferation and mineralization of MC-3T3 cels. METHODS:MC-3T3 cels cultured in vitro were selected. (1) After 24 hours of routine culture, cels were cultured in serum-free DMEM for another 24 hours to synchronize cel cycles. Then, the culture solution was removed, and cels were cultured in DMEM complete medium with (experimental group) or without (control group)Jiegu Qili Tablet solution. At 24 and 48 hours of culture, MTS assay was used to detect the proliferative ability of cels. (2) In the experimental group, MC3T3-E1 cels at a density of 1×105 were inoculated into 96-wel plates, and after 4 hours of culture, cels were cultured in an osteogenic induction medium. After 21 days of osteogenic induction, the mineralization of MC3T3-E1 cels was examined using alizarin red S staining. Cels in the control groups were treated without osteogenic induction. RESULTS AND CONCLUSION:Compared with the control group, the cel proliferation rate was up-regulated in the experimental group at 24 and 48 hours after culture (P < 0.05), and the number of calcium nodules was also larger in the experimental group at 21 days after osteogenic induction (P < 0.01). These findings indicate that Jiegu QiliTablet solution can up-regulate the proliferation and mineralization of MC-3T3 cels.
