siRNA lentiviral vectors carrying telomerase reverse transcriptase gene hasten astrocytes apoptosis
10.3969/j.issn.2095-4344.2015.11.013
- VernacularTitle:携带端粒酶反转录酶基因siRNA慢病毒载体对星形胶质细胞凋亡的影响
- Author:
Liang LIANG
;
Tao XU
;
Yang SONG
;
Weibin SHENG
- Publication Type:Journal Article
- Keywords:
Tissue Engineering;
Genes;
Cels;
Spinal Cord Injuries
- From:
Chinese Journal of Tissue Engineering Research
2015;(11):1707-1711
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Telomerase reverse transcriptase plays an important role in telomerase activation, and lentiviral vectors carrying human telomerase reverse transcriptase that can inhibit astrocyte expression are rarely reported to have effects on spinal cord injury. OBJECTIVE:To transfect rat astrocytes with telomerase reverse transcriptase gene lentiviral vectors and to observe the effect of telomerase reverse transcriptase gene lentiviral vectors on apoptosis of astrocytes. METHODS: Astrocytes from rats were subject to primary culture and subculture, and then transfected with siRNA lentiviral vector carrying telomerase reverse transcriptase gene (siRNA transfection group), simple lentiviral vector (lentiviral vector group) and nothing (blank group), respectively. Then, the transfection efficiency and apoptosis in different time periods after transfection were determined. RESULTS AND CONCLUSION:The transfection efficiency was up to 85%-90% after siRNA lentiviral vector and simple lentiviral vector transfection. The apoptosis rate of astrocytes in the siRNA transfection group was 50%-60% at 24-48 hours after transfection, but there was no significant change in the other two groups. These findings suggest that siRNA lentiviral vectors carrying telomerase reverse transcriptase gene can accelerate astrocytes apoptosis.
