Comparison of Different Extraction Methods of Genomic DNA of Cordia Dicholoma Seeds
10.3969/j.issn.1005-5304.2015.05.025
- VernacularTitle:破布木果基因组DNA不同提取方法比较研究
- Author:
Yun SUN
;
Jing SUN
;
Xiangyang CHEN
;
Shuge TIAN
- Publication Type:Journal Article
- Keywords:
Cordia dicholoma seeds;
extraction of genomic DNA;
CTAB method;
SDS method
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(5):91-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective To extract high quality genomic DNA of Cordia dicholoma seeds by using different methods;To provide references for researches on genomic DNA of Cordia dicholoma seeds. Methods Genomic DNA of Cordia dicholoma seeds was extracted through improved CTAB method and improved SDS method. Purity and concentration of obtained DNA were detected by spectrophotometry and agarose gel electrophoresis. Results The results of spectrophotometry showed that the purity of genomic DNA obtained through improved CTAB method was better than improved SDS method. Genomic DNA extracted through improved CTAB method was without protein and RNA pollution. The results of agarose gel electrophoresis showed that electrophoresis of genomic DNA obtained through the two methods both had the main belt. However, genomic DNA extracted through improved SDS method degraded more than improved CTAB method. Conclusion Improved CTAB method can obtain relatively high quality genomic DNA of Cordia dicholoma seeds.
