Changes of Treg/Th17 in mice of β2 glycoprotein 1 induced experimental anti-phospholipid syndrome
10.3969/j.issn.1000-484X.2015.04.007
- VernacularTitle:β2 GP1诱导的EAPS小鼠Treg／Th17细胞比率变化的研究
- Author:
Li XU
;
Junsong WANG
;
Yan YAN
;
Lei LIU
;
Heling DAI
;
Jia FU
;
Yanqiu FANG
;
Yan TAN
- Publication Type:Journal Article
- Keywords:
Anti-phospholipid antibody syndrome;
Regulatory T cells;
Th17 cells
- From:
Chinese Journal of Immunology
2015;(4):467-471
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the change of CD4+CD25+regulatory T cells and Th17 cells in mice with experimental anti-phospholipid antibody syndrome ( EAPS ) .Methods: EAPS model was established by immunizing BALB/c mice with recombinant humanβ2 glycoprotein 1 (rhβ2GP1).The levels of serum anti-β2 glycoprotein 1 (anti-β2GP1),anti-cardiolipin antibody (aCA),IL-17,IL-2,IL-6 and TGF-βwere tested by ELISA.The rate of abortion,activated partial thromboplastin time (APTT) and platelet count were also detected.Flow cytometry was applied to detect the percentages of the CD4+CD25+regulatory T cells and Th17 cells in peripheral blood mononuclear cells.Results:Compared with the control group,the levels of anti-β2 GP1,aCA,IL-17,IL-2 and IL-6 were significantly increased,the rate of abortion was increased,APTT time was prolonged and the levels of TGF-βand platelet count were de-creased in model mice (P<0.05).No significant difference was detected of percentage of Treg cells in PBMC at the eighth weeks in model group (P>0.05),but percentage of Treg cells was lower than that in control group after 12 weeks (P<0.05);the percentage of Th17 cells in model group was higher than that in control group (P<0.05).In addition,the ratio of Treg/Th17 cells was lower in model mice than that in control group.Conclusion: The imbalance of CD4+CD25 Treg/Th17 cells may participate in the pathogenesis of EAPS.
