MyD88,TRAF-6 expression of sinomenine on rheumatoid arthritis fibroblast- like synoviocytes
10.3969/j.issn.1000-484X.2015.04.011
- VernacularTitle:青藤碱对类风湿关节炎成纤维样滑膜细胞 MyD88、TRAF－6表达的影响
- Author:
Hongchang ZHANG
;
Mingxin LIU
;
Ying ZHANG
;
Enpeng WANG
;
Gang CHEN
;
Hongyuan JIANG
- Publication Type:Journal Article
- Keywords:
Sinomenine;
Rheumatoid arthritis;
Fibroblast-like synoviocytes;
Myeloid differentiation factor 88;
Tumor necrosisfactor receptor associated factor-6
- From:
Chinese Journal of Immunology
2015;(4):485-489
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate SIN ( Sinomenine) for TLR signal transduction pathway and MyD88 ( MyeloidDifferentiation Factor 88), TRAF-6 ( Tumor necrosis factor receptor associated factor-6) expression, clarifying SIN inhibit RA(Rheumatoid arthritis)-FLS(Fibroblast-like synoviocytes)proliferation leads to joint deformity of RA cartilage and subchondral bonedestruction caused by the role of mechanisms.Methods: RA-FLS cells for vitro were divided into a control group and(0.125,0.25,0.5,1 mmol/L)SIN group,within each group were detected by alkaline phosphatase(ALP)activity,to determine the best concentrationfor vitro drug;detect 0.5 mmol/L SIN group in CCK-8 method to detect cell proliferation rate;fluorescence quantitative PCR methodMyD88 SIN group and control group with 0.5 mmol/L TRAF-6 gene expression;Western blot method to detect MyD88 SIN group andcontrol group with 0.5 mmol/L TRAF-6 protein expression.Results: SIN the ALP activity of the lower than the control group,with theminimum ALP activity of 0.5 mmol/L SIN group(P<0.01).CCK 8 method,0.5 mmol/L RA-FLS cell proliferation rate SIN group wasobviously lower than the control group(P<0.01),SIN induce cell proliferation rate was highest,4 days into the plateau,after cell proliferationrate began to fall.Fluorescence quantitative PCR and Western blot method to detect,0.5 mmol/L SIN group of MyD88 andTRAF-6 gene and protein expression significantly lower than the control group,the difference was statistically significant(P<0.01). Conclusion: The SIN of TLR signalling pathways through effectively suppress the influence of RA-FLS MyD88 in the cell and theexpression of TRAF-6,this could be a treatment of RA prevent cartilage and subchondral bone damage cause joint deformity happened one of the important molecular mechanism.
