Characterization of growth performance of F1 hybrid CB6 F1 C57-ras transgenic mouse model
10.3969/j.issn.1671.7856.2015.004.004
- VernacularTitle:C57－ras 转基因小鼠模型杂交1代C B6 F1的生长发育特性
- Author:
Susu LIU
;
Xi WU
;
Shuya ZHOU
;
Chenfei WANG
;
Zexu PENG
;
Qin ZUO
;
Baowen LI
;
Zhengming HE
;
Changfa FAN
- Publication Type:Journal Article
- Keywords:
C57-ras transgenic mice;
Human c-Ha-ras gene;
CB6F1;
Growth performance
- From:
Chinese Journal of Comparative Medicine
2015;(4):18-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain the basic growth parameters of a self-established F1 hybrid CB6F1 C57-ras transgenic mouse model, and to provide basic information for commercialization of this mouse model. Methods F1 hybrid mice (CB6F1) were produced by crossing C57-ras heterozygous transgenic (c-Ha-ras+/-) male mice and wild-type BALB/cJ female mice.The average litter size, weaning rate, sex ratio, growth performance and C57-ras transgenic positive rate were recorded and analyzed.Results The average litter size was eight, weaning rate was 90%, and sex ratio was approximately in accordance with prediction.The average body weight of newborn mice was 1.73 ±0.05 g.The average body weight of 10-week old c-Ha-ras transgenic female and male mice in CB6F1 background was 24.38 ±1.74 g and 29.42 ±1.72g, respectively, which had a significant difference (P<0.01).The c-Ha-ras transgenic positive rate was 46.9%. which was in accordance with genetic rules.Conclusions The F1 hybrid mice (CB6F1) produced by crossing C57-ras heterozygous transgenic ( c-Ha-ras +/-) male mice and wild-type BALB/cJ female mice show normal growth performance and development characteristics, and it can be used for large-scale commercial supply.
