Effects of difluoromethylornithine on the cardiomyocytes hypertrophy and apoptosis induced by isoproterenol
10.3969/j.issn.1001-1978.2015.05.016
- VernacularTitle:二氟甲基鸟氨酸对异丙肾上腺素诱导的心肌细胞肥大与凋亡的影响
- Author:
Yan LIN
;
Wei XIAO
;
Bo LI
;
Li JIN
;
Guozhong WANG
;
Jun WANG
;
Yajun ZHAO
;
Changqing XU
;
Jicheng LIU
- Publication Type:Journal Article
- Keywords:
polyamine;
difluoromethylornithine;
car-diac hypertrophy;
apoptosis;
Bcl-2;
Bax
- From:
Chinese Pharmacological Bulletin
2015;(5):668-672
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effects of difluoromethy-lornithine (DFMO)on cardiomyocytes hypertrophy and apoptosis induced by isoproterenol (ISO).Methods The cardiomyocytes were divided into four groups ran-domly:Control group,ISO group,ISO+DFMO group and (ISO +DFMO +Putrescine)group.The hyper-trophic model of cardiomyocytes was induced by ISO, the cardiomyocytes were pretreated with 0.5 mmol · L-1 DFMO and 0.5 mmol·L-1 putrine,the gene ex-pression of ANP,the surface area of cardiomyocytes and the contents of LDH and MDA were measured. Apoptotic value of cardiomyocytes was observed by An-nexin V/PI,the gene and protein expressions of Bcl-2 and Bax were detected by real-time PCR and Western blot.Results Compared with ISO group,the cell sur-face area,the gene level of ANP,the apoptosis value of cardiomyocytes and the contents of LDH and MDA were decreased in ISO +DFMO group (P <0.05 ). Meanwhile,DFMO pretreatment upregulated the gene and protein expressions of Bcl-2 (P <0.05 ), in-creased the ratio of Bcl-2/Bax (P<0.05 ),downregu-lated the gene and protein levels of Bax (P<0.05 ). Conclusion The cardiomyocyte hypertrophy and ap-optosis induced by ISO can be protected by DFMO pre-treatment,which may be associated with the expression of apoptotic gene Bcl-2 and Bax.
