Study on HPLC Fingerprint of Total Flavonoids from Pollen ofBrassica campestrisL.

VernacularTitle:油菜花粉总黄酮部位HPLC指纹图谱研究
Author: Chengcheng FENG ; Sude YANG ; Shaowei YUAN ; Zhenzhong WANG ; Yuan BI ; Siyang FAN ; Yifang YANG ; Wei XIAO
Publication Type:Journal Article
Keywords: Pollen of Brassica campestrisL.; flavonoids; HPLC; fingerprint
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2015;17(3):591-595
CountryChina
Language:Chinese
Abstract: In this article, HPLC fingerprint analysis method for total flavonoids from pollen of Brassica campestris L.was established., The HPLC fingerprint was performed on Waters C18 column (250 mm × 4.6 mm, 5 μm), eluted gradiently with the mixture of acetonitrile and 0.4% phosphoric acid aqueous solution at a flow rate of 0.8 mL·min-1. The column temperature was 40℃. The detection wavelength was 320 nm. The HPLC standard fingerprint of total flavonoids from pollen of Brassica campestrisL. was established, and 16 common peaks were calibrated. The method was simple, stable, and reproducible. It could be applied for quality control of total flavonoids from pollen of Brassica campestris L.