Determination of Six Alkaloids in Crude and ProcessedRhizomaCoptidisby HPLC
10.11842/wst.2015.03.030
- VernacularTitle:HPLC法测定黄连药材及其炮制品中六种生物碱的含量
- Author:
Yong YANG
;
Weizao LUO
;
Jianbin SUN
;
Xin WANG
;
Lei HUA
;
Longyun LI
;
Yao QIN
- Publication Type:Journal Article
- Keywords:
Rhizoma Coptidis;
HPLC;
alkaloids;
content determination
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2015;17(3):596-602
- CountryChina
- Language:Chinese
-
Abstract:
An HPLC method to determine six alkaloids of the Coptidis for Chinese Pharmacopoeia of 2015 Edition was established through C18 column. The mobile phase was CH3CN-0.25 mol·L-1 NH4Ac (36:64) (containing 8 mmol·L-1 SDS and adjusting pH 9.3 with ammonia) at the flow rate of 1 mL·min-1, the detective wavelength was 270 nm and the column temperature was 35 oC. The linear ranges of jatrorrhizine hydrochloride, columbamine hydrochloride, epiberberine hydrochloride, coptisine hydrochloride, palmatine hydrochloride and berberine hydrochloride were 0.006 96-0.233, 0.004 75-0.152, 0.003 30-0.528, 0.006 31-1.010, 0.004 71-0.753, 0.017 8-2.884 μg·mL-1, respectively. The average recoveries were 99.65%, 98.59%, 98.49%, 98.66%, 98.64%, 98.63% and RSD were 0.03%、0.15%、0.21%、0.12%、0.28%、0.23%, respectively. The method is simple and accurate, and can be used to determine the contents of jatrorrhizine, columbamine, epiberberine, coptisine, palmatine and berberine in the Coptidis.
