Isolation and identification of cynomolgus monkey umbilical cord mesenchymal stem cells

VernacularTitle:食蟹猴脐带间充质干细胞的分离与鉴定
Author: Rongqing PANG ; Jie HE ; Ruisheng LI ; Jing ZHAO ; Hui ZHU ; Xiangqing ZHU ; Guangping RUAN ; Xinghua PAN
Publication Type:Journal Article
Keywords: Umbilical cord; Mesenchymal stem cells; Cynomolgus monkey; Cell culture
From: Chinese Journal of Comparative Medicine 2015;(4):66-69
CountryChina
Language:Chinese
Abstract: Objective To establish a method for isolation of cynomolgus monkey umbilical cord mesenchymal stem cells.Methods Fresh cynomolgus monkey umbilical cord was directly minced into pasty fine pieces, and the pieces were cultured in tissue flask with DMEM/F12 medium supplemented with 10% fetal bovine serum.The morphological characteristics of the resulting cells were examined, and their expression of mesenchymal cell surface markers were analyzed by flow cytometry.The multidifferentiation potential was examined in vitro, too.Results The fibroblast-like cells were successfully isolated from the fresh umbilical cord by an adherent culture procedure.These adherent cells expressed mesenchymal markers including CD29, CD44, and CD90, and also could be induced to differentiate into adipocytes, osteoblasts and chondrocytes.Conclusion Mesenchymal stem cells can be isolated from fresh cynomolgus monkey umbilical cord by using an adherent culture procedure.