Expression and significance of CHOP in cognitive dysfunction in rats during intermittent hypoxia
10.7652/jdyxb201503009
- VernacularTitle:CHOP 在间歇低氧大鼠认知功能减退中的表达及意义
- Author:
Hongyang WANG
;
Lijun DUAN
;
Yaning ZHAO
;
Guangchao HAN
;
Min ZHANG
;
Yanan WANG
;
Ling WANG
;
Jinli CAO
- Publication Type:Journal Article
- Keywords:
obstructive sleep apnea-hypopnea syndrome;
intermittent hypoxia;
apoptosis;
C/EBP homologous binding protein (CHOP);
Morris water maze
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(3):332-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression changes of C/EBP homologous binding protein (CHOP)in intermittent hypoxic rats with cognitive function decline and clinical significance.Methods We randomly divided 75 adult male Wistar rats into normal group (NC group),5% intermittent hypoxia group (5% CIH group)and 10%intermittent hypoxia group (10% CIH group),with 25 rats in each.Then the three groups were further divided into 3 d,7 d,14 d,21 d and 28 d subgroups,and each time a subgroup had 5 rats.The control group was exposed to the air while 10% CIH group and 5% CIH group were exposed to CIH for eight hours from 8:00 to 1 6:00 each day. After exposure for 3 d,7 d,14 d,21 d and 28 d,the cognitive function of rats was assessed with Morris water maze, the expression of CHOP in the hippocampus was assayed qualitatively by immunohistochemical technique,and the apoptosis of neurons was detected by TUNEL method.Results ① With prolonged hypoxia,the time of escape latency obviously prolonged,the time spent crossing the target quadrant shortened (P < 0.05 ),and the apoptosis index of hippocampal neurons in the CIH groups was increased gradually compared with those in control group (P <0.05).The above-mentioned indexes changed more significantly in 5% CIH group than in 10% CIH group (P <0.05).②.The expression of CHOP protein at each time point was increased (P <0.05 ).In 10% CIH group it reached the peak at 28 d while in 5% CIH group it decreased after it peaked at 21 d.③ The expression of CHOP in the two CIH groups was positively correlated with apoptosis index and animal escape latency time,but negatively correlated with the target quadrant time.Conclusion Intermittent hypoxia,which is likely to induce the high expression of CHOP and activation of neural CHOP mediated apoptosis,causes cognitive impairment of various degrees.
