Mitochondrial function and its correlation with age in articular chondrocytes of Kashin-Beck disease
10.7652/jdyxb201503014
- VernacularTitle:大骨节病软骨细胞线粒体功能及其与患者年龄的相关性
- Author:
Jiangtao LIU
;
Qingjiang PANG
;
Yun WANG
;
Cuiyan WU
;
Xiong GUO
- Publication Type:Journal Article
- Keywords:
mitochondrion;
age;
respiratory chain enzyme complex;
chondrocyte;
Kashin-Beck disease
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(3):357-361
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the mitochondrial function of chondrocytes in Kashin-Beck disease (KBD) patients and its correlation with age.Methods Mitochondrial function was evaluated by analyzing the activities of respiratory chain enzyme complexes and citrate synthase (CS),intracellular adenosine triphosphate (ATP)contents, changes in mitochondrial membrane potential (ΔΨm),as well as the intracellular reactive oxygen species (ROS) contents.Correlation of mitodhondrial function and age was analyzed with linear regression.Results Activities of complexes Ⅱ (P = 0.001 ),Ⅲ (P = 0.005 )and Ⅳ (P = 0.005 )were reduced in KBD articular chondrocytes compared with cells from normal controls.However,the mitochondrial mass was increased in KBD samples. Cultured KBD chondrocytes had reduced cellular ATP level (P = 0.001 )and mitochondrial membrane potential (P =0.001),but increased citrate synthase activity (P =0.04)and intracellular ROS level (P <0.001).We found no correlation between mitochondrial function and donor age either in normal or in KBD chondrocytes. Conclusion Mitochondrial function is altered in articular chondrocytes of KBD,and no correlation was found between mitochondrial function and donor age.
