Expression of nitric oxide synthase in esophageal mucosa of patients with gastroesophageal reflex disease
10.7652/jdyxb201503017
- VernacularTitle:一氧化氮合酶在胃食管反流病患者食道黏膜的表达
- Author:
Xiaoyan GUO
;
Ting WANG
;
Lei DONG
;
Bin QIN
;
Jiong JIANG
;
Chunrong REN
- Publication Type:Journal Article
- Keywords:
gastroesophageal reflux disease (GERD);
nitric oxide synthase (NOS);
nitric oxide (NO);
reflux esophagitis;
esophageal mucosa
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(3):373-377
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the expression of nitric oxide synthase (NOS ) in patients with gastroesophageal reflex disease (GERD)and healthy controls.Methods The distribution and relative protein amount of two NOS subtypes (nNOS and iNOS)were determined with immunohistological method,and their mRNA levels were measured with real-time PCR method.Results The nNOS and iNOS were mostly distributed in the cytoplasm in epithelia of esophageal mucosa.The nNOS and iNOS in reflux esophagitis (RE)were significantly higher than in non-erosive reflux disease (NERD)patients and healthy controls (P <0.05 ).The mRNA levels of nNOS and iNOS were also significantly higher in RE patients than in NERD patients and healthy controls (P <0.05).Conclusion The expressions of nNOS and iNOS were increased in the esophagus of RE patients,which may be related to the effects of NO on the onset of GERD.
