Evaluation of the hot and cold characteristics of ten antibiotics by cytological method
10.7652/jdyxb201503025
- VernacularTitle:用细胞学方法评价10种抗生素的寒热药性
- Author:
Siwen WANG
;
Jianli LIU
- Publication Type:Journal Article
- Keywords:
Chinese medicalization of western drugs;
antibiotic;
MTT assay;
SMMC7721 cell;
MCF-7 cell;
hot and cold characteristics
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(3):408-413
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the hot and cold characteristics of 10 antibiotics.Methods MTT assay was used to investigate the effects of 10 antibiotics on the growth and proliferation of cultured SMMC7721 cells and MFC-7 cells in vitro .Morphological changes were observed under the inverted microscope.Results The 10 antibiotics,namely,ampicillin,cefixime,cefpodoxime proxetil,cefaclor,cefalexin,azithromycin,clarithromycin, roxithromycin,doxycycline and oxytetracycline showed cold or cool characteristics.Morphological observation showed that cells treated with these drugs presented decreased cell density and turned round.Conclusion The results of this study demonstrated that the cytological method can be used to evaluate the hot and cold characteristics of western drugs.This simple and reliable method will promote research on Chinese medicalization of western drugs.
