Establishment and verification of a KCL22/NOD-SCID mouse transplantation tumor model of chronic myeloid leukemia
10.3969/j.issn.1005-4847.2015.02.016
- VernacularTitle:KCL22／NOD-SCID 小鼠慢性粒细胞白血病移植瘤模型的建立及其鉴定
- Author:
Liangxue WEN
;
Xin LIU
;
Hui LI
;
Ningshu HUANG
;
Zhenglan HUANG
;
Wenli FENG
- Publication Type:Journal Article
- Keywords:
Chronic myeloid leukemia;
KCL22 cells;
NOD-SCID mice;
Leukemia animal model
- From:
Acta Laboratorium Animalis Scientia Sinica
2015;(2):188-193
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential of chronic myeloid leukemia ( CML) cell line KCL22 in indu-cing leukemia in NOD-SCID mice for setting up a basis for constructing a CML mouse transplantation tumor model. Methods 2 ×107 KCL22 cells in logarithmic growth phase were injected via the tail vein into experimental NOD-SCID mice whereas PBS was injected to the mice of control group.General condition of the mice of both groups was observed.Wright staining was used to observe the changes of blood and bone marrow smears.PCR was conducted to detect the transcription level of BCR-ABL, and histology with HE staining was used to evaluate the tumor cell invasion in the liver and spleen. Results Four weeks after the injection of KCL22 cells, the mice in experimental group showed physical signs of decreased reactivity, depression, swollen hindlimb muscles and petechia on the hindlimb femur.Peripheral white blood cells ( WBC) began to increase after 5 weeks, with a significantly increased quantity compared with the control group (P<0.05).Imma-ture granulocytes could be seen in blood and bone marrow smears, and tumor cell infiltration was found in the liver and spleen.BCR-ABL was highly expressed in bone marrow cells.Survival time of the experimental mice without therapy was 70 days, significantly shorter than that in the control group ( >90 days) (P<0.05).Conclusions A NOD-SCID mouse model of CML transplantation tumor is successfully established with leukemia KCL22 cells.
