Change of TRAF6 expression in rat cerebral ischemia reperfusion injury
10.3969/j.issn.1671-8348.2015.10.006
- VernacularTitle:大鼠脑缺血再灌注损伤过程中TRAF6的表达变化
- Author:
Ping YUAN
;
Xiaoying HE
;
Xiaogang LI
- Publication Type:Journal Article
- Keywords:
tumor necrosis factor receptor-associated peptides and related proteins;
brain ischemia;
reperfusion injury;
cell death
- From:
Chongqing Medicine
2015;(10):1314-1316
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential role of tumor necrosis factor receptor-associated factor-6(TRAF6)in the rat cerebral ischema-reperfusion injury.Methods 40 healthy adult SD rats were divided into 5 groups(n=8)according to the ran-dom control principle:sham operation group,ischemia group,reperfusion 2 h group(R2 h),reperfusion 12 h group(R12 h)and reperfusion 24 h group(R24 h).The rat cerebral ischemia-reperfusion injury model was constructed.The change of TRAF6 expres-sion was examined by RT-PCR and Western-blot.Then,the immunohistochemistry was adopted to locate the TRAF6 protein.Re-sults Compared with the sham group,the expression of TRAF6 in the ischemia group and the R2 h,R12 h and R 24 h groups was obviously increased,but the difference had no statistical significance (P <0.05).TRAF6 was mainly located in the cytoplasm of neuronal cells.Conclusion Activated TRAF6 is involved in the brain cell death induced by cerebral ischemia-reperfusion.
