Study on the concentration of 17β-estradiol in BMSCs and different tissues of rats
10.3969/j.issn.1006-5725.2015.06.013
- VernacularTitle:大鼠骨髓间充质干细胞及不同组织中17β－雌二醇的表达情况
- Author:
Junling WANG
;
Simin HUANG
;
Qiushi WEI
;
Xin TAN
;
Weimin DENG
- Publication Type:Journal Article
- Keywords:
Osteoporosis;
17β-estradiol;
Bone marrow stem cells
- From:
The Journal of Practical Medicine
2015;(6):905-907
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the concentration of 17β-estradiol in bone marrow stem cells (BMSCs) and different tissues of rats, and study the function of estradiol produced in extragonadal sites prelimi-narily. Methods (1) The concentration of 17β-estradiol in lysate of BMSCs and in supernatant cell culture media were detected by Elisa method after rats BMSCs were serum-free cultured in 0 h , 24 h and 48 h respective ly . (2) The tissues of organs were grinded and broken under ultrasonic wave , then washed and weighed. The con-centration of 17β-estradiol in different tissues was detected by Elisa method. Results Compared with the concen-tration of 17β-estradiol at 24 h, the concentration of 17β-estradiol at 48h significantly increased (P < 0.05). The concentration of 17β-estradiol among different tissues were higher, but the difference is not significant (P > 0.05). Conclusions (1) 17β-estradiol can be secreted by BMSCs , and the concentration is proportional to the time to some degree. But whether the extragonadal estrogen can function locally is still unclear. (2) The concentration of 17β-estradiol is non- gonad dependent but whether it is secreted locally remains to be elucidated. The non- gonad estrogen could be the estrogen source of bone metabolism in order to sustain bone health after menopause.
