Photo-toxic effects of indocyanine green based on photodynamic therapy on vascular endothelial cells in ;vitro
10.3969/j.issn.1006-5725.2015.06.014
- VernacularTitle:基于光动力疗法的吲哚菁绿体外血管内皮细胞光毒性
- Author:
Shaobo ZHOU
;
Haifeng HONG
;
Juan TAN
;
Hui LI
- Publication Type:Journal Article
- Keywords:
Indocyanine green;
Photodynamic therapy;
Vascular endothelial cell
- From:
The Journal of Practical Medicine
2015;(6):908-911
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the photo-toxic effects of indocyanine green (ICG) based on photodynamic therapy (PDT) on vascular endothelial cells in vitro. Methods Human umbilical vein endothelial cells were incubated with ICG with different concentrations in 96-well plate. According to the dose of ICG , they were divided into 6 groups as 0, 50, 100, 150, 200 and 250 μmol/L group. Each group consists of 8 wells, 4 of them were irradiated with a 810 nm laser of total power 24.0 J/cm2. Cell viability was measured by MTT assay 24 h after light irradiation. Morphological changes in cells were observed by light microscope. Results With the irradiation of 810 nm light , there was a significant decrease in relative cell viability in all groups except 0 μmol/L group (all P < 0). With the increase of concentration of ICG, the cell viability dropped rapidly (P < 0). Apoptotic changes can be seen in all groups except 0 μmol/L group and necrosis was the main change in cells of 200 μmol/L and 250 μmol/L group. Conclusion PDT with appropriate concentration of ICG has a strong inhibition to vascular endothelial cells in vitro and ICG-PDT may be a good new therapy for nevascularization.
