The effect of miR-21 on the secretions of IFN-γ and IL-17 from PBMC in type 1 diabetic patients
10.3969/j.issn.1006-5725.2015.06.019
- VernacularTitle:miR鄄21对1型糖尿病患者外周血单个核细胞分泌IFN鄄γ与IL鄄17的影响
- Author:
Hui GUO
;
Jianghua LIU
;
Guojuan LI
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus,type 1;
miR-21;
Peripheral blood mononuclear cells;
IFN-γ;
IL-17
- From:
The Journal of Practical Medicine
2015;(6):925-927
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of miR-21 on the secretion of IFN-γ and IL-17 from peripheral blood mononuclear cells (PBMC) in type 1 diabetic patients (T1D). Methods The expression levels of miR-21 in PBMC from 60 T1D patients and controls by quantitative real-time quantitative polymerase chain reaction (qRT-PCR) were detected and the secretions of IFN-γ and IL-17 from T1D PBMC after transfection with miR-21 were detected by enzyme linked immunosorbent assay. Results The expression of miR-21 in PBMC from T1D patients was significantly lower than the control (P<0.01). The levels of IFN-γand IL-17 from T1D PBMC after transfection with miR-21 were remarkably downregulated compared to the control (P < 0.05 or P < 0.01). Conclusion Compared with that at the healthy control group, the expression of miR-21 in PBMC from T1D patients is decreased. MiR-21 could suppress the secretions of IFN-γand IL-17 from PBMC in T1D patients.
