Effects of RNA-binding protein La on migration and invasion of cervical cancer cells
10.7652/jdyxb201503012
- VernacularTitle:RNA 结合蛋白 La 对宫颈癌细胞侵袭转移的影响及其机制
- Author:
Qian CHEN
;
Kunlun CHEN
;
Wei LIU
;
Mu LI
;
Qing GAO
- Publication Type:Journal Article
- Keywords:
RNA-binding protein;
invasion;
cervical cancer;
RNA interference
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(3):345-348
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of RNA-binding protein La protein in the invasion and migration of cervical cancer cells.Methods RNAi technology was used to silence the La protein in HeLa cell,a cell line of cervical cancer,and then screened by G418.Finally the stably expressed HeLa-shLa cell line was constructed and then wound healing,Transwell,Western blot and gelatin zymography assay were performed. Results After La protein HeLa was silenced by RNAi,the invasion and migration capabilities of HeLa cells were decreased significantly compared with those of the controls.Meanwhile,SiRNA-mediated depletion of La reduced the expression of MMP-2 and increased the expression of TIMP-2.Meanwhile the activity of MMP-2 was reduced too.Conclusion RNA-binding protein La promotes the invasion and migration of cervical cancer cells,which may be related to regulating its matrix metalloproteinases and inhibitors.
