Effect of abnormal glucose metabolism on the expression of macrophage migration inhibitory factor and JNK in the rat liver
10.3969/j.issn.1005-4847.2015.02.012
- VernacularTitle:糖代谢异常对大鼠肝脏组织中巨噬细胞移动抑制因子及 C-Jun 氨基端激酶表达水平的影响
- Author:
Linhua FAN
;
Jianxin LIU
;
Dan LI
;
Bingyan WEI
;
Maolin LIU
;
Chunfang WANG
;
Tianfu LIU
- Publication Type:Journal Article
- Keywords:
Macrophage migration inhibitory factor;
C-Jun N-terminal kinase;
Caspase-3;
Impaired glucose toler-ance;
Type 2 diabetes mellitus;
Rat
- From:
Acta Laboratorium Animalis Scientia Sinica
2015;(2):165-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression changes of macrophage migration inhibitory factor( MIF) and C-Jun N-terminal kinase ( JNK) in the liver of rat with abnormal glucose metabolism and explore the pathological mecha-nism of abnormal glucose metabolism with non-alcoholic fatty liver disease ( NAFLD) .Methods Sixty SD rats were ran-domly divided into impaired glucose tolerance (IGT) model group (n=20), type 2 diabetes mellitus (T2DM) model group (n=20), IGT control group (n=10) and T2DM control group (n=10).IGT models were produced by feeding high-fat diet, T2DM models were produced by feeding high-fat diet for 4 weeks and intraperitoneally injected streptozotocin. Liver cell apoptosis was detected by TUNEL staining.The expression of MIF mRNA in liver tissue was determined by real-time PCR.The expressions of MIF, caspase-3, JNK proteins and phosphorylated JNK(p-JNK)in the liver tissue were de-termined by Western blotting.Results Apoptotic cells were obviously increased in the IGT and T2DM groups.Expression of MIF mRNA in the IGT and T2DM groups was markedly higher than that in the control group, respectively (P<0.01). The expressions of MIF, caspase-3, JNK proteins and phosphorylated JNK were markedly higher than that of the control group, respectively (P<0.05 or P<0.01).The expressions of MIF, caspase-3, JNK proteins in the T2DM group were significantly decreased compared with those of the IGT group, while the expression of phosphorylated JNK was significantly higher ( P<0.01) .Conclusions Abnormal glucose metabolism accompanied with NAFLD is probably related to the in-crease of MIF, Caspase-3, JNK and phosphorylated JNK expression.
