Expression and significance of SR-protein-specific kinase SRPK2 in mouse testis
10.3969/j.issn.1005-4847.2015.02.013
- VernacularTitle:丝氨酸／精氨酸蛋白特异激酶 SRPK2在小鼠睾丸组织中的表达及意义
- Author:
Hong YANG
;
Xiaobin LI
;
Ting LI
;
Sansan JIA
;
Hailong WANG
;
Guohua SONG
;
Rui GUO
- Publication Type:Journal Article
- Keywords:
SRPK2;
Spermatogenesis;
Mouse;
Testis
- From:
Acta Laboratorium Animalis Scientia Sinica
2015;(2):171-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression characteristics of SRPK2 ( serine /arginine-rich protein specific kinase 2 mRNA and its encoded protein products in mouse testis, and to reveal its molecular role during spermatogenesis. Methods Using semi-quantitative reverse transcription polymerase chain reaction ( RT-PCR) and Western blotting to an-alyze the expression of SRPK2 mRNA and its protein products in several mouse tissues.The stage-specific expression pat-tern of SRPK2 mRNA in mouse testis was examined by real-time quantitative PCR.Immunohistochemical staining was ap-plied to identify the SRPK2 protein distribution in mouse testicular seminiferous tubules and indirect immunofluorescence staining was used to detect the subcellular localization of SRPK2 protein in spermatic cells.Results Semi-quantitative RT-PCR and Western blotting analysis revealed that SRPK2 was highly expressed in mice testis.Real-time quantitative PCR analysis showed that SRPK2 mRNA was expressed abundantly at the stage of 5-and 8-week old mouse testes, suggesting that SRPK2 gene has stage-specific expression patterns during mouse spermatogenesis.Immunohistochemical and indirect immu-nofluorescence staining demonstrated that SRPK2 protein was located around the surface of elongated sperm nucleus. Conclusions SRPK2 is highly expressed in mouse testis and has significant stage-specific expression characteristics with distinct nuclear localization.These results indicate that SRPK2 may participate in precursor mRNA splicing during mouse spermiogenesis.The molecular mechanism of SRPK2 is yet to be further studied.
