Comparison of Lewis lung cancer cells and their derived cells
10.3969/j.issn.1005-4847.2015.02.015
- VernacularTitle:Lewis 肺癌细胞与其衍生细胞系的比较
- Author:
Fei WANG
;
Juan TAN
;
Youde CAO
- Publication Type:Journal Article
- Keywords:
Lewis lung carcinoma;
Orthotopic transplantation model;
Green fluorescence protein;
Mouse
- From:
Acta Laboratorium Animalis Scientia Sinica
2015;(2):182-187
- CountryChina
- Language:Chinese
-
Abstract:
Objective By comparing the biological characteristics among Lewis lung cancer cells ( LLC) , LLC or-thotopic transplantation derivative cells ( R1-LLC) and R1-LLC orthotopic transplantation derivative cells ( R2-LLC) , we e-valuate their invasion and metastatic abilities in orthotopic transplantation models.Methods In vitro, we applied CCK8, clonogenic assay, and Transwell invasion assay to detect the proliferation ability, invasion ability and morphologic structures of LLC,R1-LLC, R2-LLC cells respectively, meanwhile observing morphologic structures by transmission electron microsco-py.In vivo, we constructed LLC, R1-LLC and R2-LLC orthotopic transplantation models.Herein, we observed their tumor growth and metastasis.The tumor formation rate and tumor-forming time were recorded for statistic analysis.Results In vitro:LLC, R1-LLC and R2-LLC cells showed no significant difference in proliferation ability ( P>0.05 ) , but significant differ-ences in invasion ability:R2-LLC>R1-LLC>LLC(P<0.05).In vivo:In the orthotopic group, the tumor formation rates of LLC, R1-LLC and R2-LLC cells were 66.67%、80%、93.33%(P <0.05).Conclusions In orthotopic implantation mouse models, R2-LLC cells present higher invasion and metastatic ability than R2-LLC and LLC cells.
