Expression changes of miR-100 in maturation and cancerous rat liver

VernacularTitle:大鼠肝脏发育成熟及癌变时miR-100的表达
Author: Xiaohui JI ; Hui ZHANG ; Jinhua YANG ; Wuling ZHU
Publication Type:Journal Article
Keywords: liver neoplasm; miR-100; RT-PCR; FISH
From: Chinese Journal of Clinical and Experimental Pathology 2015;(3):306-308
CountryChina
Language:Chinese
Abstract: Purpose To explore the effect of miR-100 in rat liver development and the occurrence of liver cancer. Methods The liv-er tissues of neonate SD rats, adult SD rats and 2-AAF/PH rats were collected, then real-time fluorescence quantitative polymerase chain reaction ( RT-PCR) and FISH methods were used to detect the expression of miR-100 in the liver tissues. Results The expres-sion level of miR-100 in adult SD rats liver tissues was significantly higher than that in neonate SD rats liver tissues (P<0. 05). Com-pared with the adult rats, the expression level of miR-100 in 2-AAF/PH rats liver tissues was obviously decline (P<0. 05). Conclu-sion miR-100 can as a maker of liver mature, and its deletion maybe related to the tumor formation.