Comparative study of ALK antibody with manual and automatic immunohistochemical detec-tion in non-small cell lung cancer
10.13315/j.cnki.cjcep.2015.03.007
- VernacularTitle:手工免疫组化检测非小细胞肺癌 ALK蛋白表达的对比分析
- Author:
Qin SHEN
;
Xuan WANG
;
Bo YU
;
Biao LIU
;
Yan XU
;
Yanfen WANG
;
Qiuyuan XIA
;
Xiaojun ZHOU
- Publication Type:Journal Article
- Keywords:
lung neoplasm;
non-small cell lung cancer;
ALK;
immunohistochemistry;
screening
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(3):268-272
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the immunohistochemical ( IHC) expression of ALK antibodies with different clones in anaplastic lym-phoma kinase ( ALK) gene fusion non-small cell lung cancer ( NSCLC) . Methods ALK expression in 60 NSCLCs were detected by IHC including autostainer (D5F3, Ventana+BenchMark) and manual staining using 4 different antibodies of D5F3 (Ventana), D5F3 (Cell Signaling), 1A4/1H7 (OriGene), 5A4 (Abcam), and all cases were verified with ALK FISH. Their expressions with 4 anti-bodies were compared with those by D5F3 (Ventana+BenchMark). Results 32 ALK gene rearrangement NSCLCs and 28 negative cases were identified by FISH and D5F3 (Ventana+BenchMark). The sensitivity of D5F3 (Ventana), D5F3 (Cell Signaling), 1A4/1H7 (OriGene), 5A4 (Abcam) was 93. 8%, 84. 4%, 93. 8%, 56. 3%, and all the speciticity was 100%. The consistency with D5F3 (Ventana+BenchMark) was 96. 7%, 91. 7%, 96. 7% and 76. 7%, respectively. The validity of immunohistochemical staining in surgical resection specimens was better than in small biopsies. Conclusion Effective routine manual immunohistochemistry with high-affinity antibody clone may provide a more economic and widespread pre-screening technique.
