Genomic and phylogenetic analysis of porcine mitochondrial genomes of Rongshui miniature pig

VernacularTitle:融水小型猪线粒体 DNA 结构和系统进化的分析
Author: Jiayue XU ; Hehe SHI ; Xumin WANG ; Xiang GAO ; Xiyong YU ; Jun YU ; Xiaojiang TANG
Publication Type:Journal Article
Keywords: Rongshui miniature pig ( RMP ); Mitochondrial genome; Phylogenetic analysis; Genetic diversity
From: Chinese Journal of Comparative Medicine 2015;(3):28-34
CountryChina
Language:Chinese
Abstract: Objective Assembly whole mitochondrial genome sequence of Rongshui miniature pig ( RMP ) breed and analysis the structure of mitochondrion based on the next-generation sequecing method.Comparison of phylogenetic relationship and genetic diversity among different pig breeds.Methods We collected peripheral venous blood sample from RMP and constructed two paired-end sequencing libraries.A whole-genome shotgun sequencing strategy and Illumina Genome Analyser sequencing technology were used in our study.Results The mitochondrial genome of RMP consists of 13 protein coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and the length of pig is 16888 bp.The GC content of this pig mitochondrial genome is about 44 %.Based on phlogenetic analysis, population genetic analysis, our findings confirmed that the ancestral cluster in East Asia mainly occurred among Diannan 7#pig, Hainan wild boar, Lanyu and RMP.Conclusion RMP, a typical miniature pig breed in China, is an earlier ancestor than Lanyu pig breed.