An efficient method which can be used to transfect Tibetan minipig embryonic fibroblasts
10/3969.j.issn.1671.7856.2015.003.12
- VernacularTitle:一种高效转染西藏小型猪胚胎成纤维细胞的方法
- Author:
Wei LIU
;
Yan CHEN
;
Min YUE
;
Jin YUAN
;
Tianwu QIU
;
Dong XIAO
;
Weiwang GU
- Publication Type:Journal Article
- Keywords:
Tibetan miniature pigs;
Embryonic fibroblasts;
Transfection;
Nucleofection;
Lipofection
- From:
Chinese Journal of Comparative Medicine
2015;(3):64-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To transfect EGFP gene to porcine embryonic fibroblasts ( PEFs) of Tibetan miniature pigs by Lonza Nucleofector II machine and compare the tansfection efficiency between this method and the lipofection method. Method A plasmid carrying green fluorescent protein ( GFP) was transfected into PEFs of Tibetan miniature pigs via the Lonza Nucleofector II machine ( program U020) and by Lipofectamine 2000.Results 5 hours after nucleofection, green fluorescence was observed, indicating 80%transfecting efficiency in the nucleofection group, which is significantly higher than the lipofection group. Conclusion Nucleofector II machine can efficiently transfect PEFs, provides a reliable method for efficiently generate transgenic Tibetan minipigs.
