Effects of RNAi-mediated Cofilin-1 Gene Silencing on proliferation and invasiveness in hepatocellular carci-noma Huh-7 cells
- VernacularTitle:RNA 干扰 Cofilin-1基因沉默对人肝癌细胞株 Huh-7侵袭和转移能力的影响
- Author:
Jianping CAO
;
Xiaolan LONG
;
Yong GONG
;
Xiaojie LI
;
Hailong XIE
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Cofilin-1;
Gene
- From:
Journal of Medical Postgraduates
2015;(5):465-469
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract ] Objective Cofilin-1 is involved in the pathogenesis of various tumours .However, the expression and effect of Cofilin-1 in hepatocellular carcinoma is not clear .The aim of this study is to observe the Cofilin-1 gene expression in human hepatocel-lular carcinoma (HCC) tissues, and to explore the effect of Cofilin-1 gene expression on invasion and metastasis of HCC HuH-7 cells. Methods Real-time quantitative PCR was used to assess the Cofilin-1 gene expression in human HCC tissues and normal tumor-ad-jacent tissues.The specific small interfering RNA ( siRNA) of Cofilin-1 sequence was synthetized in vitro , and was transfected into HCC HuH-7 cells using liposome transfection.The experiment was divided into Cofilin-1-siRNA group, Ctrl-siRNA group and un-transfected group.Western blot assay was used to detect the protein expression of Cofilin-1.Migration and invasion experiments in vitro were used to investigate the invasive ability of transfected cells. Results Compared with the adjacent liver tissue , Cofilin-1 gene ex-pression in human liver cancer tissue was significantly increased (0.698 ±0.156 vs 3.523 ±0.412, P<0.05).The expression of Cofilin-1 protein in Cofilin-1-siRNA group was 0.558 ±0.033, which was lower than that in Ctrl-siRNA group ( 0.933 ±0.015 )
and in untransfected group (0.961 ±0.020) (P<0.05).The results of migration and invasion experiments in vitro showed that the amount of migration and invasion cells in Cofilin-1-siRNA group were significantly lower than Ctrl-siRNA group or untransfected group (58.50 ±1.78 vs 79.00 ±1.33, 74.50 ±1.35,P<0.05; 36.50 ±0.83 vs 60.20 ±1.60, 51.50 ±1.14, P<0.05). Conclusion Cofilin-1 is highly expressed in HCC, and the invasion and metastasis of HCC HuH-7 cells is suppressed by inhibiting the Cofilin-1 gene expression.
