Ghrelin attenuates renal fibrosis and apoptosis of obstructive nephropathy
10.3969/j.issn.1006-5725.2015.07.019
- VernacularTitle:脑肠肽改善梗阻性肾病中肾脏纤维化和细胞凋亡的实验研究
- Author:
Guangxi SUN
;
Lipei FAN
;
Xianghua SHI
;
Min LI
;
Ming ZHAO
- Publication Type:Journal Article
- Keywords:
Renal interstitial fibrosis;
Ghrelin;
Transforming growth factor beta1;
Smad3;
Apoptosis
- From:
The Journal of Practical Medicine
2015;(7):1102-1106
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and underlying mechanisms of ghrelin in a rat model of renal fibrosis. Methods Male Sprague-Dawley rats were divided into 4 groups , including sham operation +saline or brain gut peptide treatment group , model + saline or brain gut peptide treatment group. Unilateral ureteral obstruction (UUO) was established by left ureteral ligation. 7 days and 14 days after operation, the rats were sacrificed , while the kidney tissue of obstruction side was harvested for pathlogical changes through Masson coloration. Expression of α-smooth muscle actin (α-SMA), transforming growth factor beta1 (TGF-β1) and phosphorylated Smad3 (p-Smad3) in renal tissues were analyzed through immunohistochemistry. Expression of α-SMA and TGF-β1 mRNA was detected by real-time-PCR. Apoptosis kidneys cells were marked with TUNEL. Results Ghrelin inhibited renal fibrosis by reducing the production of collagen , restraining extracellular matrix (ECM) deposition and decreasing the expression of α-SMA. Meanwhile, ghrelin inhibited the accumulation of myofibroblasts by blocking the transforming growth factor-β1/Smad3 (TGF-β1/Smad3) signaling pathway. Moreover, ghrelin could attenuate renal tubular cell apoptosis induced by UUO injury. Conclusion Ghrelin can reduce renal fibrosis and renal cell apoptosis induced by UUO , demonstrating that ghrelin is a potent antifibrotic agent that may have therapeutic potential for patients with obstructive nephropathy.
