Effects of Yiqi Huayu Formula on Nitric Oxide and Nitric Oxide Synthase in Rat Models with Postpartum Subinvolution of Uterus
10.3969/j.issn.1005-5304.2015.06.017
- VernacularTitle:益气化瘀方对产后子宫复旧不全大鼠一氧化氮和一氧化氮合酶的影响
- Author:
Xiuli ZHANG
;
Jing ZENG
;
Shuibing FU
;
Jie TANG
- Publication Type:Journal Article
- Keywords:
subinvolution of uterus;
Yiqi Huayu Formula;
NO;
NOS;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(6):58-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the action mechanism of Yiqi Huayu Formula for postpartum subinvolution of uterus. Methods Fifty female SD rats were selected model with postpartum subinvolution of uterus was made by injecting escherichia coli in vagina. Rats were randomly divided into 5 groups according to pregnancy time:blank group, model group, Xinshenghua group, and Yiqi Huayu high and low dose groups. Blank group and model group received gavage with water;Yiqi Huayu low and high dose groups received gavage with Yiqi Huayu Formula;Xinshenghua group received gavage with Xinshenghua Granule, once a day for 7 days. Uterine endometrial morphology was observed by HE staining. The contents of NO and NOS were respectively detected by Nitrate reductase method. Results Uterus submucosal hyperemia, bleeding, and endometrial discontinuity with the infiltration of inflammatory cells of rats in the model group suggested that the models were successfully established. Compared with blank group, the contents of NO and NOS in model group were higher significantly. Compared with model group, the contents of NO and NOS significantly decreased in both Xinshenghua group and Yiqi Huayu high and low groups (P<0.01, P<0.01). Compared with Xinshenghua group, Yiqi Huayu high dose group was lower (P<0.05). Conclusion Yiqi Huayu Formula could promote reconstruction and repair of the endometrium by reducing the contents of NO and NOS and inhibiting abnormal uterine spiral arteries relaxation.
