Establishment and Cluster Analysis of UPLC-MS Fingerprint of Shuanghuanglian Powder-injection
10.3969/j.issn.1005-5304.2015.06.026
- VernacularTitle:双黄连粉针剂UPLC-MS指纹图谱的建立及聚类分析
- Author:
Yuanyuan GU
;
Dianming LI
;
Hongying XU
;
Dazhong CHEN
- Publication Type:Journal Article
- Keywords:
Shuanghuanglian powder-injection;
fingerprint;
UPLC;
cluster analysis
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(6):91-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the UPLC fingerprint of Shuanghuanglian powder-injection. Methods Acquity UPLCTM BEH C18 Column (2.1 mm × 50 mm, 1.7 μm) was established;mobile phase was acetonitrile and 0.1%formic acid with gradient elution;the flow rate was 0.3 mL/min;the column temperature was 40 ℃. Characteristic spectrum cluster of 13 batches of Shuanghuanglian powder-injection were analyzed, and Chinese Medicine Chromatographic Fingerprint Evaluation System (2004 A) was used to evaluate their quality. Results UPLC fingerprint common mode of 13 batches of Shuanghuanglian powder-injection was established. There were total 16 common spectrum peaks in the reference for comparison, and three main peaks were identified with better separation. The 13 batchs had good consistency, and the process was stable. Conclusion The method is rapid, efficient and can be used for full control of the quality of Shuanghuanglian powder-injection.
