Effect of ginkgolide B on TLR4 and inflammatory protein expression in high glucose treated human umbilical vein endothelial cells
10.3969/j.issn.1001-1978.2015.05.010
- VernacularTitle:银杏内酯B抑制高糖诱导内皮细胞TLR4及炎症蛋白表达
- Author:
Wenjia SUN
;
Jie SUN
;
Beidong CHEN
;
Yanyang ZHAO
;
Ruomei QI
- Publication Type:Journal Article
- Keywords:
ginkgolide B;
TLR4;
PAF receptor;
en-dothelial cells;
high glucose;
Akt;
NF-κB
- From:
Chinese Pharmacological Bulletin
2015;(5):636-640
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of ginkgolide B on TLR4 expression in glucose-treated endothelial cells.Methods Human umbilical vein endothelial cells (HUVECs)were stimulated by high concentra-tion of glucose.TLR4,inflammatory protein expression and Akt phosphorylation were analyzed by Western blot.Transcription factor NF-κB nuclear translocation was analyzed by immunofluorescence.Results The expression of TLR4 and PAF receptor was increased in high glucose-treated HUVECs. In contrast, both ginkgolide B and CV3988 dose-dependently decreased TLR4 and PAF receptor expression in high glucose-treated cells,respectively.Ginkgolide B decreased in-flammatory protein ICAM-1 ,VCAM-1 expression.Mo-reover,ginkgolide B potently abolished Akt phospho-rylation and NF-κB p65 nuclear translocation.Conclu-sion Ginkgolide B can reduce TLR4,PAF receptor, ICAM-1 and VCAM-1 expression in high dose of glu-cose-treated HUVECs,the mechanism might be linked to inhibition of Akt phosphorylation and NF-κB activa-tion.
