Expression of Indian hedgehog protein and Runt related transcription factor 2 in rats with osteoarthritis caused by anterior cruciate ligament transection
10.3969/j.issn.2095-4344.2015.18.006
- VernacularTitle:前交叉韧带离断骨关节炎大鼠印第安刺猬蛋白以及Runt相关转录因子2的表达
- Author:
Yisong SUN
;
Yunfeng YAO
;
Dong FANG
;
Juehua JING
- Publication Type:Journal Article
- Keywords:
Osteoarthritis;
Cartilage;
Anterior Cruciate Ligament;
Cartilage,Articular
- From:
Chinese Journal of Tissue Engineering Research
2015;(18):2820-2824
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Indian hedgehog homolog (Ihh) protein and its signal protein Gli1, as wel as Runt related transcription factor 2 (Runx2) are closely related to the pathogenesis of osteoarthritis. The increased expression of these factors is one of the major causes of degenerative joint changes.
OBJECTIVE:To explore the roles of Ihh, Gli1 and Runx2 in the development of osteoarthritis.
METHODS:Thirty Sprague-Dawley rats were randomly divided into two groups:control group (n=10) and model group (n=20). In the model group, rats received unilateral anterior cruciate ligament transection, to establish osteoarthritis model. Ten experimental rats were kil ed at 4 and 12 weeks after surgery respectively. Another 10 rats received unilateral knee arthrotomy as pseudo-operation controls and 10 pseudo-operation rats were kil ed at 12 weeks after surgery.
RESULTS AND CONCLUSION:In the model group, cartilage degeneration was obvious at 4 weeks and became severer at 12 weeks after anterior cruciate ligament transection operation. Expression levels of Ihh, Gli1 and Runx2 in the cartilage were increased significantly at 4 weeks after operation, but decreased at 12 weeks after operation. Experimental findings indicate that Ihh, Gli1 and Runx2 play important roles in the development of osteoarthritis, and their expression levels are significantly increased at early stage of osteoarthritis, which can be regarded as the indicators in the prophylaxis and treatment research of osteoarthritis.
