Changes of cytokines in the aqueous humor following Avastin intravitreous injection in early diabetic retinopathy rats
10.3969/j.issn.2095-4344.2015.18.026
- VernacularTitle:早期糖尿病视网膜病变模型大鼠玻璃体腔注射安维汀后房水细胞因子变化
- Author:
Jinan XIAO
;
Qingyu BAI
;
Anming XIE
- Publication Type:Journal Article
- Keywords:
Eye;
Retinal Diseases;
Diabetes Mel itus;
Aqueous Humor;
Cytokines
- From:
Chinese Journal of Tissue Engineering Research
2015;(18):2933-2939
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies on the inhibitory mechanism of Avastin for early diabetic retinopathy rats are mostly confined to the vascular endothelial growth factor, while connective tissue growth factor and pigment epithelium-derived factor also play an important role in the disease.
OBJECTIVE:To evaluate the changed cytokines of aqueous humor and significance of Avastin intravitreous injection in early diabetic retinopathy rats.
METHODS:Rat models of early diabetic retinopathy were established by inducing with streptozotocin for 10 weeks. Avastin (1.25 mg and 2.5 mg) and physiological saline were injected in the vitreous space.
RESULTS AND CONCLUSION:Enzyme linked immunosorbent assay results revealed that compared with the physiological saline injection group, mass concentration of vascular endothelial growth factor was reduced, mass concentrations of pigment epithelium-derived factor and connective tissue growth factor was increased in aqueous humor of the two Avastin injection groups (P<0.05), but no significant difference in concentration of above cytokines was detected between the two groups (P>0.05). Results verified that Avastin intravitreous injection decreased vascular endothelial growth factor levels, decreased pigment epithelium-derived factor mass concentration and increased connective tissue growth factor levels in rat models of early diabetic retinopathy.
