Bone morphogenetic protein 9-induced osteogenic differentiation of dental follicle cells in vitro
10.3969/j.issn.2095-4344.2015.14.021
- VernacularTitle:骨形态发生蛋白9体外诱导牙囊细胞的成骨分化
- Author:
Yan WU
;
Lan HUANG
- Publication Type:Journal Article
- Keywords:
Dental Sac;
Stem Cel s;
Bone Morphogenetic Proteins;
Cel Differentiation
- From:
Chinese Journal of Tissue Engineering Research
2015;(14):2255-2260
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Bone morphogenetic protein 9 is proved to promote the osteogenic differentiation of various kinds of stem cel s, but whether it can induce the osteogenic differentiation of dental fol icle cel s in vitro is yet unclear. OBJECTIVE:To investigate whether bone morphogenetic protein 9 can induce the osteogenic differentiation of rat dental fol icle cel s in vitro. METHODS:Purified rat dental fol icle cel s at passage 3 were transfected with bone morphogenetic protein 9 adenovirus. Then, alkaline phosphatase activity, calcium deposition and expression of osteogenesis-related factors at mRNA and protein levels were detected in the dental fol icle cel s. RESULTS AND CONCLUSION:After transfection with bone morphogenetic protein 9, the dental fol icle cel s showed continuously enhanced alkaline phosphatase activities and obviously enhanced calcium deposition. Real-time PCR results demonstrated that the mRNA expressions of alkaline phosphatase, osteocalcin, bone sialoprotein, osteopontin and core binding factor were increased significantly. The western blot assay showed that the expression of osteopontin enhanced in the dental fol icle cel s after transfection with bone morphogenetic protein 9. In summary, bone morphogenetic protein 9 can induce the osteogenic differentiation of dental fol icle cel s.
