Optimization of Purification Technology for Total Flavonoids from Callicarpa nudiflora Hook. et Arn. by Macroporous Adsorption Resin
10.3870/yydb.2015.05.020
- VernacularTitle:大孔吸附树脂纯化裸花紫珠总黄酮的工艺优选
- Author:
Yong LIU
;
Pengwei ZHANG
;
Wenqin SU
;
Xiao LIU
;
Keming GONG
;
Ni XIANG
- Publication Type:Journal Article
- Keywords:
Callicarpa nudiflora Hook. et Arn.;
Total flavonoids;
Macroporous Adsorption Resin
- From:
Herald of Medicine
2015;(5):640-643
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize purification technology of total flavonoids in Callicarpa nudiflora Hook. et Arm. by macroporous adsorption resin. Methods Macroporous resin models including AB-8, D-101, HP-20, HP2MG, were optimized by static adsorption and desorption experiments regarding to adsorption rate and desorption rate of total flavonoids. Purification technology parameters of total flavonoids were optimized by single factor test. Results HP-20 macroporous resin presented the best purification efficiency,the optimum purification conditions were that taking 4. 46 mg·mL-1 of total flavonoidsat pH 3. 0, loading at 3 BV·h-1, washed with 3BV of water at 3 BV·h-1,then eluted with 4 BV 75% ethanol at 2 BV·h-1, finally obtaining the total flavonoids from the dry extract of Callicarpa nudiflora Hook. et Arn. with the purity of 47. 4%. Conclusion HP-20 macroporous resin is suitable for preliminary purification of total flavonoids in Callicarpa nudiflora Hook. et Arn.
