Pre-column Derivatization HPLC Fingerprint of Mushroom Polysaccharides
10.3870/yydb.2015.05.022
- VernacularTitle:香菇多糖柱前衍生化高效液相色谱指纹图谱研究
- Author:
Zhihui CHEN
;
Lirong TAN
;
Ming LUO
;
Qimin HUANG
;
Meiling ZHANG
;
Gang WEI
- Publication Type:Journal Article
- Keywords:
Mushroom polysaccharides;
Monosaccharide;
Pre-column derivatization;
Chromatography,high performance liquid;
Fingerprint chromatogram
- From:
Herald of Medicine
2015;(5):649-654
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the chromatographic fingerprint of mushroom polysaccharides by 1-phenyl-3-methyl-5-pyrazolone( PMP) pre-column derivatization. Methods The mushroom polysaccharides was extracted by hot distilled water, precipitated by alcohol, and hydrolyzed into monosaccharides by Trifluoroacetic Acid (TFA). The hydrolysate was derivatized with 1-phenyl-3-methyl-5-pyrazolone ( PMP ) and tested via HPLC to study the monosaccharide components in mushroom polysaccharides. Results Fingerprint was established with 13 common peaks, 6 peaks in which were identified as mannose, D-glucuronic acid, D-glucose, galactose, xylose and L-fucose. The glucose accounted for the most, followed by mannose, galactose and fucose. Conclusion Development of fingerprint chromatogram by HPLC is a stable, simple, and repeatable way, which can be applied to the quality control of mushroom polysaccharides.
