Expression of hypoxia-inducible factor 1 and neuroglobin in piglet cortex during deep hypothermic circulatory arrest
10.3969/j.issn.1000-4718.2015.05.010
- VernacularTitle:深低温停循环下幼猪脑皮质HIF-1与 NGB 的表达及作用
- Author:
Mengya LIANG
;
Zhixian TANG
;
Guangxian CHEN
;
Jian RONG
;
Gang DAI
;
Zhongkai WU
- Publication Type:Journal Article
- Keywords:
Deep hypothermic circulatory arrest;
Selective antegrade cerebral perfusion;
Hypoxia-inducible factor 1;
Neuroglobin
- From:
Chinese Journal of Pathophysiology
2015;(5):823-827
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the expression of hypoxia-inducible factor 1 (HIF-1) and neuroglobin (NGB) in piglet cortex during deep hypothermic circulatory arrest.METHODS:Wuzhishan piglets were randomly assigned to car-diopulmonary bypass group ( CPB group) , 40 min of circulatory arrest ( CA) at 18 ℃ without cerebral perfusion ( DHCA group) or with selective antegrade cerebral perfusion ( SACP group) .After 180 min of reperfusion, cortical tissue was har-vested for determining HIF-1αand NGB expression by HE staining, Western blot and real-time PCR.RESULTS:Severer cerebral injury was observed in DHCA group than that in SACP group.After 180 min of reperfusion, HIF-1αprotein and mRNA levels were significantly higher in DHCA group than those in CPB group (P<0.05).Accordingly, SACP animal had higher levels of HIF-1αprotein and mRNA than those in DHCA group (P<0.05).Simultaneously, higher NGB pro-tein and mRNA levels were found in DHCA group than those in CPB group after 180 min of reperfusion ( P<0.05) .The SACP animal had higher levels of NGB protein and mRNA than those in DHCA group (P<0.05).CONCLUSION:Up-regulation of HIF-1 and NGB are involved in the mechanism against cerebral injury resulting from DHCA in the cortex and possibly a part of cerebral protective effect of SACP.
