Triggering receptor expressed on myeloid cells 2 in synovial tissue of rheumatoid arthritis rats
10.3969/j.issn.2095-4344.2015.18.004
- VernacularTitle:类风湿关节炎模型大鼠滑膜组织中证实有髓样细胞诱发受体2的表达
- Author:
Pei YE
;
Jianhua LI
;
Jinhuang XU
;
Shenghui HUANG
;
Guiwang LIU
;
Weiqiong ZHANG
;
Peizhong ZHENG
;
Jianrong HUANG
- Publication Type:Journal Article
- Keywords:
Tissue Engineering;
Arthritis,Rheumatoid;
Arthritis,Experimental;
Synovial Membrane;
Cytokines
- From:
Chinese Journal of Tissue Engineering Research
2015;(18):2807-2813
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Triggering receptor expressed on myeloid cel s 2 (TREM-2) is highly expressed throughout the synovial tissue in active rheumatoid arthritis patients, but the role of TREM-2 in the pathogenesis of rheumatoid arthritis stil remains unclear.
OBJECTIVE:To explore the TREM-2 expression in the synovial tissue of col agen type II-induced arthritis rats.
METHODS:The col agen-induced arthritis models were established in rats. The activity indicators and pathological changes of arthritis synovial were dynamical y observed. The mRNA levels of TREM-2, tumor necrosis factor-α, interleukin-1β, and interleukin-10 were detected in synovial tissue of rats by RT-PCR. The protein expression and location of TREM-2 were measured with western blot assay and immunohistochemistry, respectively.
RESULTS AND CONCLUSION:At day 13 after immunization, the paws of model rats appeared red and swel ing, the arthritis index scores were increased (P<0.01). At day 19-25 after immunization, the inflammation reached the peak. Hematoxylin-eosin staining showed that, the synovium of col agen-induced arthritis rats were proliferated and were infiltrated by inflammatory cel s, cartilage was destroyed. Compared with the control group, the expression of TREM-2 mRNA and protein, the mRNA levels of tumor necrosis factor-αand interleukin-1βin synovial tissue of the model rats were significantly increased (P<0.05 or P<0.01), while interleukin-10 mRNA expression was significantly decreased (P<0.05). Experimental findings indicate that, TREM-2 is a crucial inflammatory regulator and the increasing expression of TREM-2 plays an important role in the pathogenesis of col agen-induced arthritis.
